A. tetragonophylla phyllodes are used by Aborigines to remove warts by inserting half a dozen pungent phyllode tips in and under the wart which may be removed in four to five days. A skin wash may be prepared from the root bark. The bark gave positive tests for alkaloids and the results of phytochemical screening are also given, Barr et al. (1988).

The seeds of A. tetragonophylla are used by the Aborigines for food after grinding to a paste, Isaacs (1987).

Shurcliff (1986) indicates that the seed of A. tetragonophylla is eaten by birds in the far north-west of South Australia but does not say which birds are involved.

Davies (1976, 1978) indicates that A. tetragonophylla pods were of significant importance in the diet of emus in Western Australia.

Five species of mistletoe have been recorded on A. tetragonophylla in the State Herbarium. The most common is Amyema preissii, wire-leaved mistletoe with A. maidenii pale leaved mistletoe and A. quartdang, grey mistletoe. The wide ranging Lysiana exocarpi, harlequin mistletoe is also common with a single record of L. murrayi, mulga mistletoe.